Coding

Part:BBa_K3008004:Design

Designed by: Shayan Vahdani   Group: iGEM19_UCSC   (2019-10-06)


CAHS 106094


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Changed CAG to CAA to remove PstI site (position 264); Changed TTC to TTT to remove EcoRI site (position 453); Changed CAG to CAA to remove PvuII site (position 549).

Source

This sequence is from the genome of Hypsibius dujardini. It was codon-optimized for optimal expression in E. coli through a program written by the Silver lab at Harvard. It was sent to us in the pET-28b(+) plasmid.

References